20x concentrated Saline-Sodium-Phosphate-EDTA hybridization buffer for Northern and Southern blotting, 0.2 µm sterile-filtered, steam sterilized

0.02 M EDTA, 2.98 M NaCl in 0.2 M phosphate buffer, pH = 7.4.

SSPE Buffer 20x is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.